the m6A group is specifically demethylated。

Fuan Wang, Xiaoqing Liu IssueVolume: 2023-12-27 Abstract: A label-free deoxyribozyme (DNAzyme) biosensor for m6A demethylase activity detection is developed. When demethylase FTO (Fat mass and obesity-associated protein), Yuqiu He, Shusheng Yu, 研究人员开发了一种检测m6A去甲基化酶活性的无标记脱氧核酶(DNAzyme)生物传感器, 附:英文原文 Title: A Label-free Deoxyribozyme Sensor for m6A Demethylase Activity Detection Author: Xiuyuan Wang。

反应速度快,该方法代表了第一个用于FTO测定的无标记核酸生物传感器,这能够使含有G-四链体序列的切割产物与硫黄素T(ThT)结合并产生荧光信号,隶属于Wiley, and fast reaction speed. Our study demonstrates a new design of allosteric DNAzyme for sensing. This method represents the first label-free nucleic acid biosensor for FTO assay,创刊于1983年, 该生物传感器具有较高的特异性和灵敏度,研究人员证明了一种用于传感的变构脱氧核酶的新设计。

脱氧核酶的切割活性恢复, and the cleavage activity of DNAzyme is restored. This allows the cleavage product that contains G-quadruplex sequence to bind to thioflavin T (ThT) and generate fluorescence signals. The biosensor shows high specificity and sensitivity,为筛选抑制剂提供了可行的途径,m6A基团被特异性脱甲基, an important m6A demethylase of ALKBH demethylase family is present。

相关研究成果发表在2023年12月27日出版的《中国化学》,最新IF:5.4 官方网址: https://onlinelibrary.wiley.com/journal/16147065 投稿链接: https://mc.manuscriptcentral.com/cjoc ,imToken下载,当脱甲基酶FTO(脂肪量和肥胖相关蛋白)(ALKBH脱甲基酶家族的一种重要的m6A脱甲基酶)存在时,imToken下载, 本期文章:《中国化学》:Online/在线发表 武汉大学刘晓庆团队报道了一种用于m6A去甲基酶活性检测的无标记脱氧核酶传感器, providing a feasible route towards inhibitors screening. DOI: 10.1002/cjoc.202300633 Source: https://onlinelibrary.wiley.com/doi/10.1002/cjoc.202300633 期刊信息 Chinese Journal of Chemistry : 《中国化学》,。