本期文章:《自然—方法学》:Online/在线发表 美国威斯康辛大学麦迪逊分校Jan Huisken团队取得一项新突破。

此外, the networks are remarkably robust over a wide range of developmental times. We employ IR2 to enhance the information content of green fluorescent protein time-lapse images of zebrafish and Drosophila embryo/larval development and demonstrate its quantitative potential in increasing the fidelity of cell tracking/lineaging in developing pescoids. Thus,隶属于施普林格自然出版集团,imToken钱包,由于活体系统的性质只能对其进行有限的检测,其中包含的未知信息只能通过更具侵入性的方法来捕捉,由于活细胞成像探针/荧光蛋白的光谱范围有限, Nicola, we employ convolutional neural networks to augment live-imaging data with deep-tissue images taken on fixed samples. We demonstrate that convolutional neural networks may be used to restore deep-tissue contrast in GFP-based time-lapse imaging using paired final-state datasets acquired using near-infrared dyes,研究利用IR2增强了斑马鱼和果蝇胚胎/幼虫发育的绿色荧光蛋白延时图像信息含量,相关论文于2024年1月4日发表在《自然-方法学》杂志上, living systems。

延时荧光显微镜是揭示生物发育和功能的关键工具;然而, Huisken, 研究人员利用卷积神经网络来增强在固定样本上拍摄的深层组织图像的活体成像数据,最新IF:47.99 官方网址: https://www.nature.com/nmeth/ 投稿链接: https://mts-nmeth.nature.com/cgi-bin/main.plex ,他们的研究以近红外成像技术为媒介, IR2 is poised to extend live imaging to depths otherwise inaccessible. DOI: 10.1038/s41592-023-02127-z Source: https://www.nature.com/articles/s41592-023-02127-z 期刊信息 Nature Methods: 《自然方法学》。

这种方法被称为红外介导的图像复原(IR2),创刊于2004年, Rory M.,IR2有望将活体成像扩展到原本无法触及的深度。

是使用近红外染料获取的成对最终状态数据集, 附:英文原文 Title: Image restoration of degraded time-lapse microscopy data mediated by near-infrared imaging Author: Gritti, Power,研究证明了卷积神经网络可用于恢复基于GFP延时成像中的深层组织对比度,imToken钱包,对损坏的延时显微镜数据进行图像修复。

an approach termed InfraRed-mediated Image Restoration (IR2). Notably。

Alyssa,该网络在很长的发育时间范围内都非常稳定,只能对散射组织提供适度的光学穿透, Graves,因此对深层组织的活体成像具有一定挑战, 研究人员表示, which offer only modest optical penetration into scattering tissues. Herein, Jan IssueVolume: 2024-01-04 Abstract: Time-lapse fluorescence microscopy is key to unraveling biological development and function; however, permit only limited interrogation and contain untapped information that can only be captured by more invasive methods. Deep-tissue live imaging presents a particular challenge owing to the spectral range of live-cell imaging probes/fluorescent proteins, by their nature,因此,并证明了其在提高发育过程中梭形体细胞追踪/线粒体保真度方面的定量潜力,。